Brand New Study: Spike Protein Accumulates in Brain and Skull and Causes Damage

ABSTRACT
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), has been associated mainly with a range of neurological symptoms, including brain fog and brain tissue loss, raising concerns about the virus’s acute and potential chronic impact on the central nervous system. In this study, we utilized mouse models and human post-mortem tissues to investigate the presence and distribution of the SARS-CoV-2 spike protein in the skull-meninges-brain axis. Our results revealed the accumulation of the spike protein in the skull marrow, brain meninges, and brain parenchyma. The injection of the spike protein alone caused cell death in the brain, highlighting a direct effect on brain tissue. Furthermore, we observed the presence of spike protein in the skull of deceased long after their COVID-19 infection, suggesting that the spike’s persistence may contribute to long-term neurological symptoms. The spike protein was associated with neutrophil-related pathways and dysregulation of the proteins involved in the PI3K-AKT as well as complement and coagulation pathway. Overall, our findings suggest that SARS-CoV-2 spike protein trafficking from CNS borders into the brain parenchyma and identified differentially regulated pathways may present insights into mechanisms underlying immediate and long-term consequences of SARS-CoV-2 and present diagnostic and therapeutic opportunities.



SARS-CoV-2 Spike Protein Accumulation in the Skull-Meninges-Brain Axis: Potential Implications for Long-Term Neurological Complications in post-COVID-19 | bioRxiv
https://www.biorxiv.org/content/10.1101/2023.04.04.535604v1

SARS-CoV-2 Spike Protein Accumulation in the Skull-Meninges-Brain Axis: Potential Implications for Long-Term Neurological Complications in post-COVID-19 | bioRxiv
https://www.biorxiv.org/content/10.1101/2023.04.04.535604v1.full

The S1 protein of SARS-CoV-2 crosses the blood–brain barrier in mice | Nature Neuroscience
https://www.nature.com/articles/s41593-020-00771-8...

Frontiers | Persistence of SARS CoV-2 S1 Protein in CD16+ Monocytes in Post-Acute Sequelae of COVID-19 (PASC) up to 15 Months Post-Infection
https://www.frontiersin.org/articles/10.3389/fimmu.2021.746021/full?trk=public_post_comment-text

Persistent Circulating Severe Acute Respiratory Syndrome Coronavirus 2 Spike Is Associated With Post-acute Coronavirus Disease 2019 Sequelae | Clinical Infectious Diseases | Oxford Academic
https://academic.oup.com/cid/article/76/3/e487/6686531?login=false

Brain tissue expression of RHOA - Summary - The Human Protein Atlas
https://www.proteinatlas.org/ENSG00000067560-RHOA/brain

Molecular basis of the structure and function of H1 hemagglutinin of influenza virus - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410141/

Circulating Spike Protein Detected in Post–COVID-19 mRNA Vaccine Myocarditis | Circulation
https://www.ahajournals.org/doi/10.1161/CIRCULATIONAHA.122.061025#d1e1289