Mechanism of Gram staining - Dr. Mobeen Syed
Put bacteria on plate (if from another plate then dilute agar first);
Warm the plate to fix bacteria;
Add gram stain; 20-30 seconds; wash it;
Add iodine (mordant)
Add drops of alcohol, to wash out the gram negative bacteria’s color - 20 - 40 seconds;
Put safranin (counter stain) 20-40 seconds
What Happens?
Go to TC 2:57 in the video for illustration of how it happens:
What happens to the gram stain?
And that is how gram staining works!
Learning objectives of this video are the following:
1. Review of the procedure of gram staining.
2. Results of gram staining in case of gram positive and gram negative bacteria.
3. How gram staining works?
Presented by Dr. Mobeen Syed
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2 Comments
naveedkashif7@*.com
Jan 13 2023, 8:16 pm
Very nice and informative
attaulhadiii@*.com
Feb 14 2020, 1:22 am
Which method is used for staining mycobacteria?